Rubrique 1

maj 29122025

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HAL : Dernières publications

  • [hal-02680393] Survey of natural populations of Trichogramma (Hym., Trichogrammatidae) in the vineyards of Alsace (France)

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    ano.nymous@ccsd.cnrs.fr.invalid (O. Barnay) 01 Jun 2020

    https://hal.inrae.fr/hal-02680393v1
  • [hal-02643311] Spatial and temporal genetic variation in Tunisian field populations of <em>Macrosiphum euphorbiae</em> (Thomas)

    The potato aphid Macrosiphum euphorbiae (Thomas) is a major pest of several economic crops in Tunisia. Using 10 microsatellites,we analyzed five populations of &lt;em&gt;M. euphorbiae&lt;/em&gt; sampled during the 2004Ð2005 solanaceous and cucurbit season (April through September) from five geographic origins. From 235 aphids, 61 different multilocus genotypes were identified of which three genotypes MLG1, MLG2, and MLG42 were predominant on all host plants and regions. MLG1 and MLG2 genotypes were detected in 2004 and did not reappear in spring 2005, while the genotype MLG42 was detected only in 2005. All populations showed significant deviation from HardyÐWeinberg equilibrium even in data sets including one individual per genotype.X2 independence tests and analysis of molecular variance showed no significant differentiation among populations collected on different host plant and from different geographic origin, but differences between populations from two successive years were significant. Factorial component analysis corroborates these results. The probable causes of this seasonal variation were discussed.

    ano.nymous@ccsd.cnrs.fr.invalid (F. Raboudi) 28 May 2020

    https://hal.inrae.fr/hal-02643311v1
  • [hal-02681235] A single molecular marker to distinguish between strains of Trichogramma cacoeciae

    A single microsatellite marker was used to distinguish between strains of Trichogramma cacoeciae Marchal collected from the carob moth ( Ectomyelois ceratoniae) in Tunisia and from the grapevine moth ( Lobesia botrana) and a hawk moth in France. This marker can be used as an identification and monitoring tool in pest management programs.

    ano.nymous@ccsd.cnrs.fr.invalid (Jeannine Pizzol) 01 Jun 2020

    https://hal.inrae.fr/hal-02681235v1
  • [hal-02676088] Characterization of polymorphic microsatellite loci in the aphid species Macrosiphum euphorbiae (Hemiptera : Aphididae)

    Fourteen microsatellite loci were isolated and characterized in the potato aphid, Macrosiphum euphorbiae Thomas, by screening a genomic library with the oligonucleotide probes (GA)(10) (GT)(10) and (GATA)(4). Allelic diversity was estimated in samples collected from potato fields in Tunisia. Ten loci displayed polymorphism that ranged from two to four alleles per locus and the observed heterozygosity ranged from zero to one. These markers could be used to study the population genetic structure of this polyphagous aphid species.

    ano.nymous@ccsd.cnrs.fr.invalid (Faten Raboudi) 31 May 2020

    https://hal.inrae.fr/hal-02676088v1
  • [hal-02659527] Development of microsatellite markers for the barley stem gall midge, Mayetiola hordei (Diptera : Cecidomyiidae)

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    ano.nymous@ccsd.cnrs.fr.invalid (Maha Mezghani-Khemalhem) 30 May 2020

    https://hal.inrae.fr/hal-02659527v1
  • [hal-02656680] Genotypic diversity of the cotton-melon aphid Aphis gossypii (Glover) in Tunisia is structured by host plants

    The study of intraspecific variation with respect to host plant utilization in polyphagous insects is crucial for understanding evolutionary patterns of insect-plant interactions. Aphis gossypii (Glover) is a cosmopolitan and extremely polyphagous aphid species. If host plant species or families constitute selective regimes to these aphids, genetic differentiation and host associated adaptation may occur. In this study, we describe the genetic structure of A. gossypii collected in six localities in Tunisia on different vegetable crops, on citrus trees and on Hibiscus. The aim was to determine if the aphid populations are structured in relation to the host plants and if such differentiation is consistent among localities. The genetic variability of A. gossypii samples was examined at eight microsatellite loci. We identified only 11 multilocus genotypes among 559 individuals. Significant deviations from Hardy-Weinberg equilibrium, linkage disequilibria and absence of recombinant genotypes, confirmed that A. gossypii reproduces by continuous apomictic parthenogenesis. Genetic differentiation between localities was not significant, whereas a strong differentiation was observed between host plant families (0.175&lt;FST&lt;0.691). The great majority of aphids exhibited one of three predominant multilocus genotypes that were repeatedly and respectively associated to the three plant families, Cucurbitaceae, Solanaceae and Rutaceae, demonstrating host specialization in A. gossypii. These specialized genotypes were simultaneously found with other clones on Hibiscus, suggesting that this perennial host could act as a refuge plant between two vegetable crop seasons.

    ano.nymous@ccsd.cnrs.fr.invalid (Kamel Charaabi) 30 May 2020

    https://hal.inrae.fr/hal-02656680v1
  • [hal-02666559] Ecological specialization of the aphid Aphis gossypii Glover on cultivated host plants

    Many plant-feeding insect species considered to be polyphagous are in fact composed of genetically differentiated sympatric populations that use different hosts and between which gene flow still exists. We studied the population genetic structure of the cotton-melon aphid Aphis gossypii that is considered as one of the most polyphagous aphid species. We used eight microsatellites to analyse the genetic diversity of numerous samples of A. gossypii collected over several years at a large geographical scale on annual crops from different plant families. The number of multilocus genotypes detected was extremely low and the genotypes were found to be associated with host plants. Five host races were unambiguously identified (Cucurbitaceae, cotton, eggplant, potato and chili- or sweet pepper). These host races were dominated by asexual clones. Plant transfer experiments using several specialized clones further confirmed the existence of host-associated trade-offs. Finally, both genetic and experimental data suggested that plants of the genus Hibiscus may be used as refuge for the specialized clones. Resource abundance is discussed as a key factor involved in the process of ecological specialization in A. gossypii.

    ano.nymous@ccsd.cnrs.fr.invalid (Jerome Carletto) 31 May 2020

    https://hal.inrae.fr/hal-02666559v1
  • [hal-02670991] Comparison between the control efficacy of Trichogramma evanescens Wetswood (Hymenoptera : Trichogrammatidae) and two Trichogramma cacoeciae Marchal strains against grapevine moth (Lobesia botrana Den. Schiff.), depending on their release density

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    ano.nymous@ccsd.cnrs.fr.invalid (Gerard Hommay) 31 May 2020

    https://hal.inrae.fr/hal-02670991v1
  • [hal-02691565] Characterization of a microsatellite locus in the parasitoid wasp Aphelinus abdominalis (Hymenoptera : Aphelinidae)

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    ano.nymous@ccsd.cnrs.fr.invalid (Flavie Vanlerberghe Masutti) 01 Jun 2020

    https://hal.inrae.fr/hal-02691565v1
  • [hal-02766351] Metapopulation structure in Aphis gossypii in greenhouses : evidence from molecular markers

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    ano.nymous@ccsd.cnrs.fr.invalid (Flavie Vanlerberghe Masutti) 04 Jun 2020

    https://hal.inrae.fr/hal-02766351v1
  • [hal-02776349] RAPD fingerprinting of aphid populations sampled in Citrus orchards

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    ano.nymous@ccsd.cnrs.fr.invalid (Flavie Vanlerberghe Masutti) 04 Jun 2020

    https://hal.inrae.fr/hal-02776349v1
  • [hal-02688680] Characterization of a highly conserved satellite DNA from the parasitoid wasp Trichogramma brassicae

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    ano.nymous@ccsd.cnrs.fr.invalid (I. Landais) 01 Jun 2020

    https://hal.inrae.fr/hal-02688680v1
  • [hal-02689023] Host-based genetic differentiation in the aphid Aphis gossypii Glover, evidenced from RAPD fingerprints

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    ano.nymous@ccsd.cnrs.fr.invalid (Flavie Vanlerberghe Masutti) 01 Jun 2020

    https://hal.inrae.fr/hal-02689023v1
  • [hal-02689264] Variation in clonal diversity in glasshouse infestations of the aphid, Aphis gossypii Glover in southern France

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    ano.nymous@ccsd.cnrs.fr.invalid (S.J. Fuller) 01 Jun 2020

    https://hal.inrae.fr/hal-02689264v1
  • [hal-02688307] Inter-strain competition and dispersal in aphids : evidence from a greenhouse study

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    ano.nymous@ccsd.cnrs.fr.invalid (J. Rochat) 01 Jun 2020

    https://hal.inrae.fr/hal-02688307v1
  • [hal-02686228] Characterization of microsatellite loci in the aphid species Aphis gossypii Glover

    [...]

    ano.nymous@ccsd.cnrs.fr.invalid (Flavie Vanlerberghe Masutti) 01 Jun 2020

    https://hal.inrae.fr/hal-02686228v1

 

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